Carolyn M. Slupsky

  • Assistant Professor, Department of Nutrition/ Department of Food Science & Technology
  • Assistant Nutritionist in Agricultural Experiment Station

3247 Meyer

(530) 219-5757

cslupsky@ucdavis.edu

Email

Education

  • Ph.D., Biochemistry, University of Alberta
  • B.Sc., Biochemistry, University of Alberta

Research Interests

Dr. Slupsky’s research interests include the application of analytical metabolomics for the purpose of studying cellular metabolism as it relates to food, nutrient intake, and health status. She predominantly uses NMR spectroscopy to collect as much chemical information as possible about the identity and concentrations of metabolites in cell cultures, tissues, and body fluids obtained from animal models and humans.

Publications

  • Slupsky CM, Shaw GS, Campbell AP, and Sykes BD. A 1H NMR study of a ternary peptide complex that mimics the interaction between troponin C and troponin I. Protein Sci1992; 1:1595-1603.
  • Slupsky CM, Kay CM, Reinach FC, Smillie LB, and Sykes BD. Calcium-induced dimerization of troponin C: Mode of interaction and use of trifluoroethanol as a denaturant of quaternary structure. Biochemistry 1995; 34:7365-7375.
  • Slupsky CM, Reinach FC, Smillie LB, and Sykes BD. Solution secondary structure of calcium-saturated troponin C monomer determined by multidimensional heteronuclear NMR spectroscopy. Protein Sci 1995;4:1279-1290.
  • Slupsky CM, and Sykes BD. NMR solution structure of  Calcium-saturated skeletal muscle troponin C. Biochemistry 1995; 34:15953 -15964.
  • Slupsky CM, Gentile LN, and McIntosh LP. Assigning the NMR spectra of aromatic amino acids in proteins: Analysis of two Ets pointed domains. Biochem Cell Biol 1998;76:379 -390.
  • Slupsky CM, Gentile LN, Donaldson LW, Mackereth CD, Seidel JJ, Graves BJ, and McIntosh LP. Structure of the Ets-1 pointed domain and mitogen-activated protein kinase phosphorylation site. Proc Natl Acad Sci USA 1998;95:12129 -12134.
  • Elisseeva EL, Slupsky CM, Crump MP, Clark-Lewis I, and Sykes BD. NMR studies of active N-terminal peptides of stromal cell-derived factor-1: Structural basis for receptor binding. J Biol Chem 2000;275:26799-26805.
  • Keizer DW, Crump MP, Lee TW, Slupsky CM, Clark-Lewis I, and Sykes BD. The human CC chemokine I-309, structural consequences of the additional disulfide bond. Biochemistry 2000;39:6053 -6059.
  • Slupsky CM, Desautels M, Huebert T, Zhao R, Hemmingsen SM, and McIntosh LP. Structure of Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe. J Biol Chem 2001;276:5943-5951.
  • Desautels M, DenHaese JP, Slupsky CM, McIntosh LP, and Hemmingsen SM. Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe, interacts with a phosphatidylinositol-4-kinase. J Biol Chem 2001;276:5932-5942.
  • Bjorndahl TC, Watson MS, Slupsky CM, Malcolm BA, Spyracopolous L, Sykes BD, and Wishart DS. Complete 1H, 13C, and 15N backbone assignments for the hepatitis A virus 3C protease. J Biomol NMR 2001;19:187-188.
  • Keizer DW, Slupsky CM, Kalisiak M, Campbell AP, Crump MP, Sastry PA, Hazes B, Irvin RT, and Sykes BD. Structure of a pilin monomer from Pseudomonas aeruginosa: Implications for the assembly of pili. J Biol Chem 2001;276: 24186-24193.
  • Slupsky CM, Sykes DB, Gay GL, and Sykes BD. The HoxB1 hexapeptide is a prefolded domain: Implications for the Pbx1/Hox interaction. Protein Sci 2001; 10:1244-1253.
  • Graether SP, Slupsky CM, Davies PL, and Sykes BD. Behavior of type I antifreeze protein and mutants in supercooled water. Biophys J 2001; 81:1677-1683.
  • Bhattacharyya S, Habibi-Nazhad B, Amegbey G, Slupsky CM, Yee A, Arrowsmith C, and Wishart DS. Identification of a novel archaebacterial thioredoxin: Determination of function through structure. Biochemistry 2002;41:4760 -4770.7
  • Raussens V, Slupsky CM, Ryan RO, and Sykes BD. NMR structure and dynamics of a receptor-active apolipoprotein E peptide. J Biol Chem 2002:277:29172-20180.
  • Graether SP, Slupsky CM, and Sykes BD. Freezing of a fish antifreeze protein results in amyloid fibril formation. Biophys J 2003;84:552-557.
  • Lakey JRT, Moser G, Helms LMH, Lix B, Slupsky CM, Rebeyka I, Sykes BD, and McGann LE. Dynamics of cryoprotectant permeation in porcine heart valve leaflets. Cell Transpl 2003;12:123 -128.
  • Raussens V, Slupsky CM, Ryan RO, and Sykes BD. Lipid-bound structure of an apolipoprotein E-derived peptide. J Biol Chem 2003; 278:25998-26006.
  • Booth V, Slupsky CM, Clark-Lewis I, and Sykes BD. Unmasking ligand binding motifs: identification of a chemokine receptor motif by NMR studies of antagonist peptides. J Mol Biol 2003;327:329 -334.
  • Slupsky CM, Boyko RF, Booth V, and Sykes B.D. SMARTNOTEBOOK: A  semi-automated assignment procedure. J Biomol NMR 2003;27:313 -321.
  • Wan X, Xu D, Slupsky CM, and Lin G. Automated protein NMR resonance assignments. Proc IEEE Comput Soc Bioinform Conf  2003;2:197-208.
  • Mackereth CD, Scharpf M, Gentile LN, MacIntosh SE, Slupsky CM, and McIntosh LP. Diversity in structure and function of the Ets family PNT domains. J Mol Biol 2004;342:1249 -1264.
  • van Schaik EJ, Giltner CL, Audette GF, Keizer DW, Bautista DL, Slupsky CM, Sykes BD, and Irvin RT. DNA binding: a novel function of Pseudomonas aeruginosa type IV pili. J Bacteriol 2005:187:1455 -1464.
  • Graether SP, Slupsky CM, and Sykes BD. Effect of a mutation on the structure and dynamics of an a-helical antifreeze protein in water and ice. Proteins 2006; 63:603-610.
  • Weljie AM, Newton J, Mercier P, Carlson E and Slupsky CM. Targeted profiling: Quantitative analysis of 1H-NMR metabolomics data. Anal Chem 2006;78:4430 -4442.
  • Saude EJ, Slupsky CM, and Sykes BD. Optimization of NMR analysis of biological fluids for quantitative accuracy. Metabolomics 20006;2:113-123.6
  • McGrath BM, Greenshaw AJ, McKay R, Slupsky CM, and Silverstone PH. Lithium treatment alters regional rat brain myo-inositol concentrations at 2 and 4 weeks: An Ex Vivo MRS study at 18.8 Tesla. NeuroReport 2006;17: 1323-1326.
  • McGrath BM, Greenshaw AJ, McKay R, Slupsky CM, and Silverstone PH. Unlike lithium, anticonvulsants and antidepressants do not alter rat brain myo-inositol. Neuroreport  2007;18:1595-1598.
  • Slupsky CM, Spyracopoulos L, Booth VK, Crump MP, and Sykes BD. Probing nascent structures in peptides using natural abundance 13C NMR relaxation and reduced spectral density mapping. Proteins 2007;67:18-30.
  • Slupsky CM, Rankin KN, Wagner J, Fu H, Chang D, Weljie AM, Saude EJ, Lix B, Adamko DJ, Shah S, Greiner R, Sykes BD, and Marrie TJ. Investigations of the effects of gender, diurnal variation, and age in human urinary metabolomic profiles. Anal Chem 2007;79:6995-7004.
  • McGrath BM, McKay R, Dave S, Seres P, Weljie AM, Slupsky CM, HanstockCC, Greenshaw AJ, Silverstone PH. Acute dextro-amphetamine administration does not alter brain myo-inositol levels in humans and animals: MRS investigations at 3T and 18.8T. Neurosci Res 2008;61:351 -359.
  • Murdoch TB, Fu H, MacFarlane S, Sydora B, Fedorak RN, Slupsky CM. Urinary metabolic profiles reveal progression of inflammatory bowel disease in interleukin-10 gene deficient mice. Anal Chem 2008;80:5524-5531.
  • Mahadevan S, Shah SL, Marrie TJ, Slupsky CM. Analysis of Metabolomic Data using Support Vector Machines. Anal Chem 2008;80:7562-7570.