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Darshan S. Kelley

Darshan S. Kelley

  • Adjunct Professor, Department of Nutrition
  • USDA ARS Western Human Nutrition Research Center Scientist

207 WHNRC

(530) 752-5138

dkelley@whnrc.usda.gov

Email

Education

  • B.Sc.,Agriculture and Animal Husbandry, Punjab Agricultural University, Ludhiana, India
  • M.Sc., (Hons) Biochemistry, Punjab Agricultural University, Ludhiana, India
  • Ph.D., Biochemistry, University of Oklahoma, Oklahoma City

Research Interests

Dr. Darshan S. Kelley is a research chemist with the Western Human Nutrition Research Center. Dr. Kelley is interested in studying the effects of diets on inflammation and immune responses. The focus of his studies has been the modulation of risk factors for cardio-vascular disease and insulin resistance by dietary fatty acids and phytonutrients. He is also interested in the effects of fatty acids on cell growth, differentiation and apoptosis.

Selected Publications

  • Kelley, D.S., Malik, N.S. and Ichhponani, J.S. (1971) Effect of Various Combinations of Vegetable and Animal Proteins on the Performance of Starter Chicks. Indian J. Anim. Sci. 41 (6): 484-489.
  • Kelley, D.S., Malik, N.S. and Ichhponani, J.S. (1971) Effect of Different Mashes on the Lipid Composition of Adipose Tissue of Chicks. Indian J. Anim. Sci. 41 (8): 710-712.
  • Chaudhary, K.C., Kelley, D.S. and Marawha, S.R. (1971) Egg and its lipids in Different Strains of Poultry. Indian Journal of Poultry Science 6 (2): 12-16.
  • Kelley, D.S., Malik, N.S. and Ichhponani, J.S. (1972) Fatty Acid Composition of Chicks' Adipose Tissue Under Different Feeding Regimens. The Indian Veterinary Journal 49 (3): 268-274.
  • Watson, J.J., Cook, C., Kelley, D.S., Chandler, A.M. and Johnson B.C. (1973). Effects of Sucrose Versus Fat as Energy Source in the Diet on Rat Liver Polysome Patterns. J. Nut. 103: 1521-1527.
  • Kelley, D.S., Watson, J.J., Mack, D.O. and Johnson, B.C. (1975) Glucose-6-Phosphate Dehydrogenase is not Induced in the Mammalian Liver by Dietary Carbohydrate. Nutrition Reports International 12 (3): 121-135.
  • Kelley, D.S. and Potter, V.R. (1977) Amino Acid Concentrating Ability of Slowly Growing Autochthonous Hepatomas and Host Livers. Biochem. Biophys. Res. Communs. 75 (2): 219-225.
  • Kelley, D.S., Becker, J.E. and Potter, V.R. (1978) Effect of Insulin, Dexamethasone and Glucagon on the Amino Acid Transport Ability of Four Rat Hepatoma Cell Lines and Rat Hepatocytes in Culture, Cancer Research. 38: 4591-4600.
  • Kelley, D.S. and Potter, V.R. (1978) Regulation of Amino Acid Transport Systems by Amino Acid Depletion and Supplementation in Monolayer Cultures of Rat Hepatocytes. J. Biol. Chem. 253: 9009-9014.
  • Kelley, D.S. and Potter, V.R. (1979) Repression, Derepression, Trans-inhibition and Trans-stimulation of Amino Acid Transport in Rat Hepatocytes and Four Rat Hepatoma Cell Lines in Culture. J. Biol. Chem. 254: 6691-6697.
  • Kelley, D.S. and Potter, V.R. (1980) Hormonal Regulation of Amino Acid Transport and cAMP Production in Rat Hepatocyte Monolayer Cultures. J. Cell Physiol.. 103: 159-168.
  • Kelley, D.S., Evanson, T. and Potter, V.R. (1980) Calcium-Dependent Hormonal Regulation of Amino Acid Transport and cAMP Accumulation in Rat Hepatocyte Monolayer Cultures. Proc. Natl. Acad. Sci., U.S.A., 77: 5953-5957.
  • Kelley, D.S., Campbell, H.A. and Potter, V.R. (1982) Effect of Hormones and Amino Acid Depletion on the Kinetic Parameters of Amino Acid Uptake in Monolayer Cultures of Rat Hepatocytes. J. Cell Physiol.. 112, 67-75.
  • Kelley, D.S. (1982) Effect of Glucagon on Amino Acid Transport and cAMP Production in Rat-Hepatoma Cell Line McA-RH 8994 in Culture. Cancer Research 42, 3116-3119.
  • Quinlan, D.C., Todderud, C.G., Kelley, D.S. and Kletzien, R.F. (1982) Sodium Gradient-Stimulated Transport of L-Alanine by Plasma-Membrane Vesicles Isolated from Liver Parenchymal cells of Fed and Fasted Rats: Crucial Role of Adrenal Glucocorticoids. Biochemical Journal. 208, 685-693.
  • Kletzien, R.F., Stumpo, D.J. Kelley, D.S. and Todderud, C.G. (1983) Primary Cultures of Hepatocytes as a Model System for Studies on the Chronic Effects of Hormones on Hepatic Carbohydrate Metabolism. In, ""Isolation, Characterization, and Use of Hepatocytes"", Elsevier Press, (eds, Harris, R., and Cornell, N.) 77-86.
  • Kelley, D.S. (1984) Hormonal and Nutritional Regulation of Amino Acid Transport and cAMP Metabolism in Normal and Malignant Hepatocytes in Culture. ""In Vitro Models for Cancer Research"" CRC Press, (eds., Webber, M.M., and Sekely, L.) 91-116.
  • Kelley, D.S. and Kletzien, R.F. (1984) Ethanol Modulation of the Hormonal and Nutritional Regulation of Glucose-6 Phosphate Dehydrogenase Activity in Primary Cultures of Rat Hepatocytes. Biochem. Jour. 217, 543-549.
  • Kelley, D.S., Nelson, G.J. and Hunt, J.E. (1986) Effect of Prior Nutritional Status on the Activity of Lipogenic Enzymes in Primary Monolayer Cultures of Rat Hepatocytes. Biochem Jour. 235, 87-90.
  • Nelson, G.J., Kelley, D.S. and Hunt, J.E. (1986) Effect of Nutritional Status on the Fatty Acid Composition of Rat Liver and Cultured Hepatocytes. Lipids 21, 454-459.
  • Kelley, D.S., Nelson, G.J. Serrato, C.M. and Schmidt, P.C. (1987) Nutritional Regulation of Hepatic Lipogenesis in the Rat. Nutrition Research. 7, 509-517.
  • Nelson, G.J., Kelley, D.S., Schmidt, P.C. and Serrato, C.M. (1987) The Effect of Fat-Free, Saturated and Polyunsaturated Fat Diets on Rat Liver and Plasma Lipids. Lipids. 22, 88-94.
  • Nelson, G.J., Kelley, D.S., Schmidt, P.C. and Serrato, C.M. (1987) The Influence of dietary Fat on the Lipogenic Activity and Fatty Acid Composition of Rat White Adipose Tissue. Lipids, 22, 338-344.
  • Kelley, D.S., Nelson, G.J., Serrato, C.M., Schmidt, P.C. and Branch, L.B. (1988) Effect of Type of Dietary Fat on the Indices of Immune Status of Rabbits. J. Nutr. 118:1376-1384.
  • Nelson, G.J., Kelley, D.S., Schmidt, P.C., Serrato, C.M. and Lindgren, F.T. (1989) Effect of Menhaden Oil and Various Seeds Oils on Serum Lipids and Lipoproteins in Rabbits. Nutr. Res. 9:531-544.
  • Kelley, D.S., Branch, L.B. and Iacono, J.M. (1989) Nutritional Modulation of Human Immune Status. Nutr. Res. 9:965-975.
  • Fraga, C.G., Tappel, A.L., Leibovitz, B.E., Kuypers, F., Chier, D., Iacono, J.M., and Kelley, D.S. (1990) Lability of Red Blood Cell Membranes to Lipid Peroxidation: Application to Humans Fed Polyunsaturated lipids. Lipids 25:111-114.
  • Kelley, D.S., Schaeffer, M.C., Sampson, D.A., Taylor, P.C., and Rivera, Y.M. (1990) The Effect of Moderate Vitamin B6 Deficiency on Immunological and Hematological Parameters of Adult Female Rats. Annals New York Academy of Sciences 587:292-293.
  • Kelley, D.S., Branch, L.B., Love, J.E., Taylor, P.C., Rivera, Y.M. and Iacono, J.M. (1991) Dietary Alpha-Linolenic Acid and Immunocompetence in Humans. Am. J. Clin. Nutr. 53:40-6.
  • Kelley, D.S., Serrato, C.M., Taylor, P.C. and Rivera, Y.M. (1991) Effect of Calcium on the Isolation and in vitro proliferation on rabbit peripheral blood mononuclear cells. Amer. Lab 10:18-20.
  • Kelley, D.S. (1991) Effect of Dietary Fatty Acids on Cell Mediated Immune System. In: Health Effects of Dietary Fatty Acids, Ed. Gary J. Nelson, Amer. Oil Chem. Soc., Champaign, IL, p 184-202.
  • Burri, B.J., Dougherty, R.M., Kelley, D.S., Iacono, J.M. (1991) Platelet Aggregation in Humans is Affected by Replacement of Dietary Linolenic Acid with Oleic Acid. Am. J. Clin. Nutr. 54:359-62.
  • Jacob, R.A., Kelley, D.S., Pianalto, R., Swendseid, M.E., Henning, S.M., Zhang, J.Z., Ames, B.E., Fraga, C.G., Peters, J.H. (1991) Immunocompetence and Oxidant Defense During Ascorbate Depletion of Healthy Men. Am. J. Clin. Nutr. 54:1302S-9S.
  • Kelley, D.S., Dougherty, R.M., Branch L.B., Taylor, P.C., and Iacono, J.M. (1992) Concentration of Dietary N-6 Polyunsaturated Fatty Acids and the Human Immune Status. Clin. Immunol. and Immunopath. 62:240-244.
  • Kelley, D.S., Nelson, G.J., Branch, L.B., Taylor, P.C., Rivera, Y.M. and Schmidt, P.C. (1992) Salmon Diet and Human Immune Status. Eur. J. Clin. Nutr. 46:397-404.
  • Kelley, D.S., (1992) Alpha-linolenic Acid and Immune Response. Nutrition, 8:215-217.
  • Kelley, D.S. and Daudu, P.A., (1993). Fat Intake and Immune Response. Progress in Food and Nutrition Science, 17:61-63.
  • Kelley, D.S., Nelson, G.J., Love, J.E., Branch, L.B., Taylor, P.C., Schmidt, P.C., Mackey, B.E. and Iacono, J.M. (1993). Dietary a-Linolenic Acid Alters Fatty Acid Composition, but not Blood Lipids, Lipoproteins or Coagulation Status in Humans. Lipids 28: 533-537.
  • Kelley, D.S., Daudu, P.A., Branch L.B., Johnson, H.L., Taylor, P.C., and Mackey, B. (1994). Energy Restriction Decreases Number of Circulating Natural Killer Cells and Serum Levels of Immunoglobulins in Overweight Women. Eur. J. Clin. Nutr. 48:9-18.
  • Daudu, P. Kelley, D.S., Taylor, P.C., Burri, B.J., and Wu, M.M. (1994). Effect of Low B-Carotene Diet on Immune Functions of Adult Women. Am. J. Clin. Nutr. 60: 969-72.
  • Kelley, D.S., Daudu, P.A., Mackey, B.E., and Turnlund, J.R. (1995). Effects of Copper Depletion on Human Immune Response. Am. J. Clin. Nutr. 62 412-416.
  • Kelley, D.S. (1995). Immunomodulatory Effects of Flax Seed and Other Oils Rich in a-Linolenic Acid In: Flax Seed in Human Nutrition, Ed. S.C. Cunnane and L.U. Thompson. American Oil Chem. Soc. Champaign, IL, p 145-156.
  • Kelley, D.S. (1996). Essential Nutrients and Immunologic Functions. Am. J. Clin. Nutr.63:994S-996S.
  • Kelley,D.S. (1996). Dietary Fat and Human Immune response. INFORM, 7: 852- 858.
  • Kelley,D.S., Taylor, P.C., Nelson, G.J., Schmidt, P.C., Mackey, B.E.(1997). Effect of Dietary Arachidonic Acid on Human Immune Response. Lipids, 32 : 449-456.
  • Nelson, G.J., kelley, D.S., Emken, E.A., Phinney, S.D., Kyle ,D, and Ferretti, A.(1997). A Human Arachidonic Acid Study Conducted in a Metabolic Research Unit: The Study Design. Lipids, 32 : 415-420.
  • Nelson, G.J., Schmidt, P.C., Bartolini, G. Kelley, D.S., Kyle, D.(1997). The Effect of Dietary Arachidonic Acid on Platelet Function, Platelet Fatty Acid Composition, and Blood Coagulation in Humans. Lipids, 32, 421-425.
  • Nelson, G.J., Schmidt, P.C., Bartolini, G., Kelley, D.S., Kyle, D., Silberman,S. and Schafer, E. (1997). The Effect of Dietary Arachidonic Acid on Lipoprotein Distributions, Apoproteins, Blood Lipids, and Fatty Acid Compostion in Humans. Lipids, 32 : 427-433.
  • Ferretti, A., Nelson, G.J., Schmidt, P.C., Kelley, D.S., Bartolini, G., and Flanagan,V.P. (1997). Effect of Dietary Arachidonic Acid on Eicosanoid Production as Measured in Urine. Lipids, 32: 435-439.
  • Kelley, D.S., Taylor, P.C., Johnson, H.L., Mackey, B.E.(1998). Energy Restriction and Immunocompetence in Overweight Women. Nutrition Research. 18:159-169.
  • Nelson, G.J., Schmidt, P.C., Bartolini, G., Kelley, D.S., Kyle, D. (1998). The Effect of Dietary Docosahexaenoic Acid on Platelet Function, Platelet Fatty Acid Composition, and Blood Coagulation in Humans. Lipids. 32: 1129-1136.
  • Nelson, G.J., Schmidt, P.C., Bartolini, G., Kelley, D.S., Kyle, D. (1998). The Effect of Dietary Docosahexaenoic Acid on Plasma Lipoprotein Distributions, Apolipoproteins, Blood Lipid Levels, and Tissue Fatty Acid Composition in Humans. Lipids 32:1137-46.
  • Ferretti, A., Nelson, G.J., Schmidt, P.C., Kelley, D.S., Bartolini, G., and Flanagan,V.P. (1998) Dietary Docosahexaenoic Acid Reduces the Thromboxane / Prostacyclin Synthesis Ratio in Humans. Nutritional Biochemistry. 9: 88-92.
  • Kelley, D.S., Taylor, P.C., Nelson, G.J., Mackey ,B.E.(1998). Arachidonic Acid Supplementation Enhances Eicosanoid Synthesis Without Suppressing Immune Functions in Humans. Lipids. 33: 125-130.
  • Kelley, D.S., Taylor, P.C., Nelson, G.J., Mackey ,B.E.(1998). Dietary Docosahexaenoic Acid and immuncompetence in young healthy men. Lipids 33: 559-566.
  • Kelley, D.S., Taylor, P.C., Nelson, G.J., Schmidt, P.C., Ferretti, A., Erickson, K., Mackey, B.E. (1998). Docosahexaenoic Acid Feeding to Healthy Men Inhibits Select Immune Cell Functions. Proceedings 10th International Congress Immunology, p 701-706.
  • Nelson, G.J., Schmidt, P.C., Bartolini, G., Kelley, D.S., Kyle, D. (1998). Dietary Docosahexaenoic Acid Lowers Plasma Triglycerides in the Absence of Dietary Eicosapentaenoic Acid in Human Males. Proceedings of Fourth International Congress on Essential Fatty Acids and Eicosanoids. AOCS Press, Champaign, IL, pp 307-324.
  • Kelley, D.S. (1999). Fatty Acids and Immune Functions. In AMilitary Strategies for Sustainment of Nutrition and Immune Function in the Field, National Academy Press, p 305-316.
  • Kelley, D.S., Taylor, P.C., Nelson, G.J., Schmidt, P.C., Ferretti, A., Erickson, K., Yu, R. Chandra, R.K., Mackey, B.E. (1999) Docosahexaenoic Acid Ingestion Inhibits Natural Killer Cell Activity and Production of Inflammatory Mediators in Young Healthy Men. Lipids 34:317-324.
  • Kelley, D. S., Rudolph I. (2000) Effect of Individual Fatty Acids of N-3 and N-6 Type on Human Immune Status and Role of Eicosanoids. Nutrition, In Press.
  • Kelley,D.S., Taylor, P.C.,Rudolph, I.L., Benito, P., Nelson, G.J., Mackey, B.E., Erickson, K., (2000)Dietary Conjugated Linoleic Acid Did Not Alter Immune Status in Young Healthywomen. Lipids 35: 1065-1071.
  • Medina EA, Horn WF, Keim NL, Havel PJ,Benito P, Kelley DS, Nelson GJ,and Erickson KL (2000). Conjugated Linoleic Acid Supplementation in Humans:Effect on Circulating Leptin Level and Appetite, Lipids 35:735-83.
  • Zambell KL, Keim KM, VanLoan M, GaleB, Benito P, Kelley DS, NelsonGJ, and Erickson KL (2000). Conjugated Linoleic Acid Supplementation in Humans:Effects on body Composition and Energy Expenditure, Lipids 35: 777-82.
  • Rudolph, I.L., Kelley, D.S., Klasing, K.C., andErickson, K.L. (2001) Regulation of Cellular Differentiation and Apoptosis byFatty Acids and Their Metabolites. Nutr. Res., 21:381-393.
  • Kelley,D.S., 2001) Modulationof Human Immune and Inflammatory Responses by Dietary Fatty Acids. Nutrition17:669-673
  • Kelley,D.S., Simon, V. A.,Taylor, P.C., Rudolph, I.L., Benito, P., Nelson, G.J., Mackey, B.E., Erickson,K., (2001) Dietary Supplementation with Conjugated Linoleic Acid Increased Itsconcentration in Human Peripheral Blood Mononuclear Cells, but Did Not AlterTheir Function. Lipids 36: 1-6.
  • Benito, P., Nelson, G. J., Kelley, D. S., Bartolini, G., Schmidt,P. C., Simon, V. (2001) The Effect of Conjugated Linoleic Acid on plateletFunction, Platelet Fatty Acid Composition, and Blood Coagulation in Humans.Lipids 36: 221-227.
  • Benito, P., Nelson, G. J., Kelley, D. S., Schmidt, P. C., Simon,V. (2001) The Effect of Conjugated Linoleic Acid on Plasma Lipoproteins andTissue Fatty Acid Composition in Humans. Lipids 36: 229-236.
  • Hawkes, W.C., Kelley, D. S., Taylor, P. C. (2001)The Effect of Dietary Selenium on the Immune System in Healthy Men.Biological Trace Elements Research 81: 189-21.
  • Kelley DS, Warren JM, Simon VA, Bartolini G,Mackey BE, and Erickson KL (2002). Similar Effects of c9, t11-CLA and t10,c12-CLA on Immune Cell Function in Mice. Lipdis37: 725-728.
  • Pinna K, Kelley DS, Taylor PC, and King JC (2002). Immune Functions areMaintained in Healthy Men with Low Zinc Intake. J Nutr. 132: 2033-2036.
  • Kelley, D.S., Erickson, K.L. (2003)Modulation of Body Composition and Immune Cell Functions by Conjugated LinoleicAcid in Humans and Animal Models: Benefits vs. Risks. Lipids 38(4):377-86.
  • Warren, J.M., Simon, V.A., Bartolini, G.,Erickson, K.L., Mackey, B.E., Kelley, D.S. (2003) Trans-10, Cis-12 CLAIncreases Liver and Decreases Adipose Tissue Lipids in Mice: Possible Roles ofSpecific Lipid Metabolism Genes. Lipids 38:497-504
  • Jacob, R.A., Spinozzi, G.M., Simon,V.A., Kelley, D.S., Prior, R.L., Hess‑Pierce, B., Kader, A.A. (2003)Consumption of Cherries Lowers Plasma Urate in Healthy Women.J Nutr. 133(6):1826‑9.
  • Kelley DS, Batolini GL, Warren JM,Mackey BE, Erickson KL (2004) Contrasting Effects of t10, c12- and c9,t11-Conjgated Linoleic Acid Isomers on the Fatty Acid Profiles of Mouse LiverLipids. Lipids 39:135-141.
  • Turnlund JR, Jacob RA, Keen CL,Strain JJ, Kelley DS, Domek JM, Keyes WR, Ensuna JL, Lykkesfeldt J, Coulter J.(2004). Long-term High Copper Intake: Effects on Indexes of Copper Status, andImmune Function in Young Healthy Men. Am J Clin Nutr 79:1037-44.
  • Hubbard NE, Kelley DS, Erickson KL (2004)Effects of Dietary Conjugated Linoleic Acid on Immune Functions and Cancer. InNutrition and Immunology for 21st Century (Chandra RK editior) TSAR Health, Toronto, pp 83-99
  • Erickson KL, Kelley DS, Hubbard NE(2004). Dietary Fat, Immunity, and Cancer. In Diet and Immune Functions (HughesDA, Darlington LG, Bendich A editors) Humana Press, Totowa, NJ, pp 345-360.
  • Erickson KL, Kelley DS, Hubbard NE(2005). Dietary Fat, and Immunity in Humans (2005) In Handbook of Nutrition andImmunity (Gershwin ME, Nestel P, Keen CL editors) Humana Press, Totowa , NJ
  • Kelley DS, Hubbard NE,Erickson KL (2005) Regulation of Human Immune and Inflammatory Responses byDietary Fatty Acids. Advances in Food and Nutrition Research. 50:101-138.
  • Kelley DS, Rasooly R, Jacob RA, Kader AA, Mackey ME (2006) Consumptionof Bing Sweet Cherries Lowers Circulating Concentrations of InflammationMarkers in Healthy Men and Women. J. Nutr. 136:981-86.
  • Kelley DS, Barolini GB, Newman JW, Vemuri V, andMackey B (2006). Fatty acidcomposition of liver, adipose tissue, spleen, and heart of mice fed dietscontaining t10, c12-, and c9, t11-conjugated linoleic acid. Prostglandins,Leukotrienes, Essential Fatty Acids 74: 331-338.
  • ReuvenRasooly, Darshan S. Kelley and JeffGreg, Bruce.E. Mackey (2007). Dietary t10, c-12-Conjugated Linoleic AcidReduces the Expression of Fatty Acid Oxidation and Drug Detoxification Enzymesin Mouse Liver. British Journal of Nutrition 97: 58-66.
  • Vemuri M, Kelley DS, andErickson KL (2007) Health effects of foods rich in polyphenols. In press WildWestern Diets.
  • Vemuri M, and Kelley DS (2007). Effect of dietary fatty acids on lipid metabolism. In: Fatty Acids inFoods and Their Health Implications, 3rd edition. CK Chow editor;Marcel and Dekker, Inc, 591-630.
  • Vemuri M, Kelley DS, and Erickson KL (2007) Health effects of foods rich in polyphenols. In: Wild-Type Food in Health Promotion and Disease Prevention; The Columbus Concept. F De Meester and RR Watson editors, Humana Press, p 393-412.
  • Vemuri M, Kelley DS, Rasooly R, Giovanni GB, Mackey BE (2007). Docosahexaenoic acid but not eicosapentaenoic acid prevents the conjugated linoleic acid induced insulin resistance in mice. Metabolic Syndrome and Related Disorders 5:315-322.,
  • Kelley DS, Siegel D, Vemuri M, Mackey BE (2007). Docosahexaenoic acid supplementation improves fasting and postprandial lipid profiles in hypertriglyceridemic men, American Journal of Clinical Nutrition 86:324-333.
  • Kelley DS, David Siegel, Madhuri Vemuri, Gloria H. Chung, and Bruce E. Mackey. (2008). Docosahexaenoic Acid Supplementation Decreases Remnant-Like Particle-Cholesterol and Increases the (n-3) Index in Hypertriglyceridemic Men. Journal of Nutrition, 138: 30–35,
  • Kelley DS, Madhuri Vemuri, Yuriko Adkins, Sher Himmat S. Gill, Dawn Fedor, Bruce. E. Mackey (2008).Flaxseed Oil Prevents Trans-10, Cis-12 Conjugated Linoleic Acid Induced Insulin Resistance in Mice. Journal of Nutrition, In review.